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KMID : 1005220080230020087
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Journal of Embryo Transfer
2008 Volume.23 No. 2 p.87 ~ p.91
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Effects of Glutamine, Glucosamine and Glutathione on the In Vitro Maturation of Porcine Oocytes
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Kim Young-Hee
Rhee Man-Hee
Kim Sang-Keun
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Abstract
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This study was carried out to investigate the effects of the supplementation of glutamine, glucosamine and glutathione on the porcine oocytes on IVM rates. Cocs were incubated in NCSU-23 supplemented with at glucosamine, glutamine and glutathione for 48 hrs. Oocytes were transferred to 50 ul drops of maturation medium covered with mineral oil and cultured in a incubator (, 5% , 95% air). The IVM rates of oocytes cultured in NCSU-23 supplemented with 0.5, 1.0, 2.0 and 4.0 mM glutamine for 48 hrs were , , and , respectively. The IVM rates of oocytes cultured in NCSU-23 supplement with 2.0, 5.0, 7.0, 10.0 mM glucosamine for 48 hrs were , , and , respectively. The IVM rates of oocytes cultured in NCSU-23 supplemented with glucosamine were no significantly increased compare to the control (). The IVM rate of oocytes cultured in NCSU-23 supplemented with 3.0, 5.0, 7.0, 10.0 mM glutathione for 48 hrs were , , , , respectively. The IVM rate of oocytes cultured in NCSU-23 supplemented with glutamine and glutathione were significantly increased co~pared to those control (). Glucosamine did not affect the IVM rates of oocytes. IVM rates of oocytes cultured in NCSU-23 medium for 48 hrs were significantly increased compared to the cultured for 40 hrs.
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KEYWORD
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porcine oocytes, glucosamine, glutamine, glutathione, IVM rate
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